ABSTRACT
Objective: To determine the role of glutathione S-transferase [GST] isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort
Subjects and Methods:In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs
Results:Of the 150 patients with psoriasis, 83 [79%] were identified with the GSTT1 genotype and 22 [21%] with the null genotype. Of the 102 patients in the control group, 69 [67.6%] subjects were identified with the GSTT1 genotype and 33 [32.4%] with the null genotype. There was no significant difference between the patient and control groups [p = 0.063]. Regarding the GSTM1 polymorphism, 54 [51.4%] patients were identified with this genotype and 51 [48.6%] with the null genotype; in the control group, 50 [49%] were identified with this genotype and 52 [51%] with the null geno- type. Again there was no statistically significant difference between the groups [p = 0.957]
Conclusion:In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed
ABSTRACT
No abstract available.
ABSTRACT
BACKGROUND: In-stent restenosis (ISR) is the major limitation of percutaneous coronary stenting procedure. The elements like nickel, chromate and molybdenum are known to cause contact allergy. Hypersensitivity reaction, against these metal ions, may be one of the reasons of ISR. Cobalt chromium coronary stents, which are increasingly being used in percutaneous coronary interventions, have more nickel amount than the stainless steel stents. OBJECTIVE: We aimed to investigate the association between nickel hypersensitivity reaction and ISR in patients treated with cobalt chromium coronary stents. METHODS: Epicutaneous patch tests for nickel were applied to 31 patients who had undergone elective cobalt chromium coronary stent implantation and had ISR in control angiogram. Thirty patients, without ISR, were included as the control group. Patch test results and other clinical variables were compared. RESULTS: There was no statistically significant difference of the mean age, sex, body mass index, rate of hypercholesterolemia, diabetes, hypertension and smoking between the patients with and without ISR. All other lesion characteristics were similar in the 2 groups. According to the patch test results, 7 patients had nickel contact allergy. All of these patients were in the ISR group, which was statistically significant (p<0.006). CONCLUSION: Patients treated with cobalt chromium coronary stents and had ISR were found to have significantly more nickel allergy than the control group. Nickel allergy may play role in restenosis pathophysiology.